23-Chem-B4 Biochemical Engineering
Worked solutions to 9 past sittings (2013–2019), 47 questions. Pick a sitting, or start from a topic below.
Topics across the sittings
Topics that come up in more than one sitting, taken from the headings of our worked solutions. A topic counts once per sitting.
- Batch (3 sittings): May 2017 Q3 · December 2014 Q4 · May 2014 Q4
- Aerobic Respiration (2 sittings): December 2014 Q5 · May 2014 Q3
- Air-On/Air-Off Method (2 sittings): May 2018 Q2 · May 2017 Q4
- Cell Immobilization (2 sittings): December 2019 Q5 · May 2018 Q5
- Continuous Productivity (2 sittings): December 2014 Q4 · May 2014 Q4
- Enzyme (2 sittings): December 2019 Q1 · May 2018 Q5
- Fat Metabolism (2 sittings): May 2016 Q5 · December 2014 Q5
- Immobilized-Enzyme Packed-Bed Column (2 sittings): May 2018 Q1 · December 2014 Q2
- Industrial Example (2 sittings): December 2019 Q5 · May 2018 Q5
- K L a (2 sittings): May 2017 Q4 · December 2016 Q4
- Limitations (2 sittings): December 2019 Q5 · May 2018 Q5
- Methods (2 sittings): December 2019 Q5 · May 2018 Q5
- Thiele Modulus (2 sittings): May 2017 Q5 · May 2016 Q1
Questions by sitting
December 2019
- Question 1: Enzymes — Definition, Classification & First-Principles Derivation of the Michaelis–Menten Equation
- Question 2: Michaelis–Menten Enzyme Reaction in a CSTR — Design-Equation Derivation & Reactor Volume for Glucose–Fructose Isomerization
- Question 3: Air-On/Air-Off Method for k L a & Principles of Continuous Sterilization
- Question 4: Product Kinetics, Washout, Maintenance Coefficient & Nutritional Requirements
- Question 5: Enzyme and Cell Immobilization — Methods, Advantages, Limitations, and an Industrial Example
May 2018
- Question 1: Immobilized-Enzyme Packed-Bed Column — Zero-Order, External- and Internal-Diffusion-Limited Sizing
- Question 2: The Air-On/Air-Off Method and the Principles of Batch Sterilization
- Question 3: Continuous HTST Sterilization — Flowsheet and Advantages Over the Batch Process
- Question 4: Approaches for Cell Disruption in Biochemical Engineering
- Question 5: Enzyme and Cell Immobilization — Methods, Advantages, Limitations, and an Industrial Example
May 2017
- Question 1: Fed-Batch Culture — Definition, Advantages & First-Principles Proof that μ=D at Quasi-Steady-State
- Question 2: Animal/Mammalian Cell Culture — Key Terms, Nutrient Media & Optimum Conditions
- Question 3: Batch vs. Continuous Sterilization of Nutrient Media
- Question 4: k L and k L a — Definitions and the Air-On/Air-Off Method
- Question 5: Dimensionless Groups in Bioreactor Systems — Re, Sh, Sc, Thiele Modulus, Damköhler Number
December 2016
- Question 1: Michaelis–Menten Enzyme Kinetics — Competitive, Non-Competitive & Uncompetitive Inhibition
- Question 2: Geometric Bioreactor Scale-Up — Four Impeller-Speed Criteria
- Question 3: HTST Sterilization — Direct Steam Injection vs. Indirect Regenerative Heating
- Question 4: The Air-On/Air-Off (Dynamic Gassing-Out) Method for q O2 and k L a
- Question 5: Nucleic Acid Structure and the Process of Protein Synthesis
May 2016
- Question 1: Immobilized-Enzyme CSTR — Haldane Kinetics, Thiele Modulus & Effectiveness Factor
- Question 2: HTST Batch Sterilization — Spore Kill vs. Heat-Labile Vitamin Destruction
- Question 3: Aerobic vs. Anaerobic Metabolism; Organelles of Eukaryotic Cells
- Question 4: Overall vs. Liquid-Film Oxygen Mass-Transfer Coefficient (Two-Film Theory)
- Question 5: Fat Metabolism and Oxygenic Photosynthesis
December 2014
- Question 1: Oxygen-Transfer Capacity of a Stirred-Tank Aerator
- Question 2: Immobilized-Enzyme Packed-Bed Column — Specific Surface Area and Effective Diffusivity
- Question 3: Photosynthesis and Fermentation Metabolism
- Question 4: Chemostat with Recycle; Batch vs. Continuous Productivity
- Question 5: TCA Cycle, Aerobic Respiration, and Fat Metabolism
May 2014
- Question 1: Bioreactor Scale-Up Criteria
- Question 2: Internal Diffusion Limitation in Immobilized Enzyme Beads
- Question 3: Prokaryotes vs. Eukaryotes; Aerobic Respiration
- Question 4: Chemostat Steady State and Batch vs. Continuous Productivity
- Question 5: Protein Structure and Eukaryotic Organelles
- Question 6: Continuous Sterilization — HTST Direct Steam Injection vs. Indirect Heating
May 2013
- Question 1: Chemostat Data – Yield Coefficient and Monod Parameters
- Question 2: Comparison of Bioreactor Configurations
- Question 3: Batch Culture Growth Kinetics
- Question 4: Metabolic Pathways and Cell Structure
- Question 5: Biomolecules and Bacterial Metabolic Types
- Question 6: Monod Growth Rate Calculation
Undated paper
- Question 1: Maximum Oxygen-Transfer Flux in a Stirred-Tank Bioreactor
- Question 2: First-Principles Derivation of the Michaelis–Menten Equation
- Question 3: Effective Diffusivity in an Immobilized-Enzyme Bead from the Observed Thiele Modulus
- Question 4: Geometric Scale-Up of a Cell-Culture Bioreactor
- Question 5: High-Temperature Short-Time (HTST) Sterilization — Two Process Configurations